Princess Serenity has long blonde hair put up in odango buns. She has blue eyes and wears a white regal gown and has a golden crescent-moon symbol on her forehead.
Serenity was fascinated by the Earth, and longed for its greenery and wind. Even though she was forbidden to go to the planet, she would go there in secret and later meet Prince Endymion. The two then fell in love.
During the Dark Kingdom's attack, the catalyst of the Moon Kingdom's downfall, Prince Endymion died protecting Serenity, and out of sorrow, she committed suicide. Serenity's mother, the Moon Queen, sealed away the evil forces of the Dark Kingdom, but as a result, the Moon Kingdom turned into stone, and Earth's history has been restarted. Before her own death, the Queen used the Silver Crystal to give her daughter and others a second chance of rebirth, hoping that this time, Serenity and Endymion could find happiness together. As a result, Serenity's spirit was reborn and reincarnated in 21st century Earth as Usagi Tsukino.