Zoisite has long wavy blonde hair pulled back in a ponytail. He wears a blue-grey suit with green trim and black boots. He has green eyes.
Zoisite apperers in episode 3, teasing Jadeite for not failing. In episode 4, Zoisite introduces himself (along with the other three Shitennou) to the Sailor Guardians.
In episode 6, Queen Beryl sends Zoisite to dearch the Silver Crystal. Disguising himself as a woman and calling himself Dr Isono, he releases energy from the screens and televisions, brainwashing people.
He fails to get the crystal again, as seen in episode 7 where his brainwashing plan is foiled by Sailor Moon. However, he does manage to knock out Mamoru, though Sailor Venus foils his plan to hurt Sailor Moon.
In Episode 10, he learns that he was a knight of Endymion's after eaves dropping on Beryl. In Episode 12, he ends up being killed, much like the rest of his comrades, but in Episode 13, he returns and appears with the rest of the Shitennou .